Protein "purity," proteoforms, and the albuminome: critical observations on proteome and systems complexity.

ABSTRACT:

Introduction

The identification of effective, selective biomarkers and therapeutics is dependent on truly deep, comprehensive analysis of proteomes at the proteoform level.

Methods

Bovine serum albumin (BSA) isolated by two different protocols, cold ethanol fractionation and heat shock fractionation, was resolved and identified using Integrative Top-down Proteomics, the tight coupling of two-dimensional gel electrophoresis (2DE) with liquid chromatography and tandem mass spectrometry (LC-MS/MS).

Results and discussion

Numerous proteoforms were identified in both "purified" samples, across a broad range of isoelectric points and molecular weights. The data highlight several concerns regarding proteome analyses using currently popular analytical approaches and what it m

SUBMITTER: Woodland B 

PROVIDER: S-EPMC11666697 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature