Arabidopsis VQ motif-containing proteins VQ1 and VQ10 interact with plastidial 1-deoxy-D-xylulose-5-phosphate synthase.

ABSTRACT: VQ1 and VQ10 are largely unstructured homologous proteins with a significant potential for protein-protein interactions. Yeast two-hybrid (Y2H) analysis confirmed that both proteins interact not only with themselves and each other but also with other VQ and WRKY proteins. Screening an Arabidopsis Y2H library with VQ1 as bait identified 287 interacting proteins. Validation of the screening confirmed that interactions with VQ1 also occurred with VQ10, supporting their functional homology. Although VQ1 or VQ10 proteins do not localize in plastids, 47 VQ1-targets were found to be plastidial proteins. In planta interaction with the isoprenoid biosynthetic enzyme 1-deoxy-D-xylulose-5-phosphate synthase (DXS) was confirmed by co-immunoprecipitation. DXS oligomerizes through redox-regulated interm

SUBMITTER: Gayubas B 

PROVIDER: S-EPMC11666741 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature