Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy.

ABSTRACT: Single-molecule localization microscopy (SMLM) can decipher fine details that are otherwise impossible using diffraction-limited microscopy. Often, the reconstructed super-resolved images suffer from noise, strong background and are prone to false detections that may impact quantitative imaging. To overcome these limitations, we propose a technique (corrSMLM) that recognizes and detects fortunate molecules (molecules with long blinking cycles) from the recorded data. The method uses correlation between two or more consecutive frames to identify and isolate fortunate molecules that blink longer than the standard blinking period of a molecule. The corrSMLM is based on the fact that random fluctuations (noise) do not last longer (usually limited to a single frame). In contrast, fortunate mole

SUBMITTER: S A 

PROVIDER: S-EPMC11666785 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature