Impacts of APOE-ε4 and exercise training on brain microvascular endothelial cell barrier function and metabolism.

ABSTRACT:

Background

The APOE-ε4 genotype is the highest genetic risk factor for Alzheimer's disease (AD), and exercise training can reduce the risk of AD. Two early pathologies of AD are degradation of tight junctions between brain microvascular endothelial cells (BMEC) and brain glucose hypometabolism. Therefore, the objective of this work was to determine how the APOE-ε4 genotype and serum from exercise trained individuals impacts BMEC barrier function and metabolism.

Methods

iPSC homozygous for the APOE-ε3 and APOE-ε4 alleles were differentiated to BMEC-like cells and used to measure barrier function and metabolism. To investigate exercise effects, serum was collected from older adults pre- and post- 6 months of exercise training (n = 9 participants per genotype). APOE-ε3 and APOE

SUBMITTER: Weber CM 

PROVIDER: S-EPMC11667009 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature