Microbiome and metabolomics reveal the effect of gut microbiota on liver regeneration of fatty liver disease.

ABSTRACT:

Background

Metabolic dysfunction-associated fatty liver disease (MAFLD) is associated with impaired regenerative capacity and poor postoperative prognosis following hepatectomy. Previous research has highlighted the importance of the gut-liver axis in the physiological and pathological processes of the liver. However, the contribution of gut bacteria to the regeneration of livers with MAFLD and its metabolic regulatory mechanisms remain elusive.

Methods

Partial hepatectomy (PHx) was performed on C57Bl/6J mice fed with high-fat diet (HFD) for 12 weeks. Pathological examination, immunohistochemistry, and qRT-PCR analysis were performed to assess the severity of steatosis and proliferative potential. The gut microbiome was examined by 16S rRNA gene sequencing and shotgun metage

SUBMITTER: Hu Y 

PROVIDER: S-EPMC11667181 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature