Bile acids in the lower airways is associated with airway microbiota changes in chronic obstructive pulmonary disease: an observational study.

ABSTRACT:

Background

Chronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in patients with COPD and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage (BAL) fluid as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes and bacterial lung colonisation.

Methods

We used BAL specimens from a cohort of patients with COPD and healthy controls. BAs were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL pellets and quantified using quantitative PCR. We profiled the BAL microbiota using an amplicon sequencing a

SUBMITTER: Caparros-Martin JA 

PROVIDER: S-EPMC11667286 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature