Design of buffer property for the new enrichment method of circulating tumor cell based on immunomagnetic-negative separation.

ABSTRACT: Metastasis is a significant contributor to cancer-related mortality and a critical issue in cancer. Monitoring the changes in circulating tumor cells (CTCs) with metastatic potential is a valuable prognostic and predictive biomarker. CTCs are a rare population in the peripheral blood of patients with cancer. The enrichment process is extremely important for the isolation of clinically significant CTC subpopulations, which can then be used for further analysis. The present study postulates that the buffer serves as an essential field for immunomagnetic separation, thereby enhancing the efficacy of CTC enrichment in peripheral blood. This, in turn, facilitates CTC detection. Here, we describe the design of buffers for developing a novel immunomagnetic-negative separation method for CTC enric

SUBMITTER: Hoshi K 

PROVIDER: S-EPMC11667567 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature