Intra- and extracellular real-time analysis of perfused fibroblasts using an NMR bioreactor: A pilot study.

ABSTRACT:

Introduction

Metabolomic discrimination of different mitochondrial defects is challenging. We describe an NMR-based bioreactor allowing real-time intra- and extracellular metabolic investigation of perfused fibroblasts.

Objectives

The objective of this study is (I) determining whether metabolic investigations of perfused fibroblasts overall and separated for intra- and extracellular contributions by real-time NMR allows for discrimination of different representative mitochondrial defects in a feasibility study and (II) gaining insight into physiological consequences of mitochondrial dysfunction in basal condition and during glycolysis inhibition.

Methods

Overall, intra- and extracellular metabolomes of malate dehydrogenase 2 (MDH2), pyruvate dehydrogenase (PDH), compl

SUBMITTER: Urzi C 

PROVIDER: S-EPMC11667653 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature