Switchback RNA.

ABSTRACT: Intricately designed DNA and RNA motifs guide the assembly of robust and functional nucleic acid nanostructures. In this work, we present a globally left-handed RNA motif with two parallel strands called switchback RNA and report its assembly, biophysical, and biochemical characterization. Switchback RNA can be assembled in buffers without Mg²⁺, with improved thermal stability in buffers containing Mg²⁺, Na⁺, or K⁺. Differences in the binding of small molecules to switchback RNA and conventional RNA indicate design-based approaches for small molecule loading on RNA nanostructures. Further, the differential affinity of the two component strands in switchback or conventional duplex conformations allows for toehold-less strand displacement. Enzyme s

SUBMITTER: Madhanagopal BR 

PROVIDER: S-EPMC11667664 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature