Novel approach to alleviate lupus nephritis: targeting the NLRP3 inflammasome in CD8⁺CD69⁺CD103⁺ T_RM cells.

ABSTRACT:

Background

Renal CD8⁺ tissue-resident memory T (T_RM) cells display prolonged survival and activity in lupus nephritis (LN), exacerbating renal pathology. NLRP3 regulates the T cell response. This study explored the impact of NLRP3 inflammasome activity on the regulatory functions of T_RM cells in LN.

Methods

NLRP3 inflammasome activity in renal CD8⁺ T_RM cells from lupus-prone MRL/lpr mice and in vitro induced human CD8⁺CD103⁺ T cells was assessed by quantifying NLRP3, caspase-1, gasdermin D (GSDMD), and IL-1β levels using flow cytometry, ELISA, and western blotting analysis. The specific NLRP3 inhibitor MCC950, caspase-1 inhibitor Ac-YVAD-cmk, and NF-κB inhibitor JSH23 were utilized to delineate the

SUBMITTER: Lai Y 

PROVIDER: S-EPMC11668076 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature