Disrupting shadow in the prothoracic gland induced larval development arrest in the fall armyworm Spodoptera frugiperda.

ABSTRACT:

Introduction

The juvenile hormone (JH) and 20-hydroxyecdysone (20E) are the central regulating hormones of insect development. The timing of their secretion usually leads to developmental transitions.

Methods

The developmental transitions were evaluated via the starvation treatment and the expressions of two key metamorphosis inducing factor in Spodoptera frugiperda. Then, the main endocrine organs, including the brain-corpora cardiacum-corpora allatum and prothoracic gland, were sampled from L4-24 h and L6-24 h larvae for the RNA-seq analysis. Additionally, the critical rate-limiting enzyme of 20E synthesis, shadow, was knocked down to mimic the downregulation of 20E synthesis in the late larval instar.

Results

The critical weight (CW), when JH titer de

SUBMITTER: Wu MZ 

PROVIDER: S-EPMC11668756 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature