Bronchoscopic biopsies - a novel source for primary airway epithelial cells in respiratory research.

ABSTRACT:

Background

Using primary airway epithelial cells (AEC) is essential to mimic more closely different types and stages of lung disease in humans while reducing or even replacing animal experiments. Access to lung tissue remains limited because these samples are generally obtained from patients who undergo lung transplantation for end-stage lung disease or thoracic surgery for (mostly) lung cancer. We investigated whether forceps or cryo biopsies are a viable alternative source of AEC compared to the conventional technique.

Methods

AECs were obtained ex vivo from healthy donor lung tissue using the conventional method and two biopsy procedures (forceps, cryo). The influence of the isolation method on the quality and function of AEC was investigated at different time-points duri

SUBMITTER: Barbet K 

PROVIDER: S-EPMC11669235 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature