Modulating Liposome Surface Charge for Maximized ATP Regeneration in Synthetic Nanovesicles.

ABSTRACT: In vitro reconstructed minimal respiratory chains are powerful tools to investigate molecular interactions between the different enzyme components and how they are influenced by their environment. One such system is the coreconstitution of the terminal cytochrome bo₃ oxidase and the ATP synthase from Escherichia coli into liposomes, where the ATP synthase activity is driven through a proton motive force (pmf) created by the bo₃ oxidase. The proton pumping activity of the bo₃ oxidase is initiated using the artificial electron mediator short-chain ubiquinone and electron source DTT. Here, we extend this system and use either complex II or NDH-2 and succinate or NADH, respectively, as electron entry points employing the natur

SUBMITTER: Deutschmann S 

PROVIDER: S-EPMC11669383 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature