Confinement-sensitive volume regulation dynamics via high-speed nuclear morphological measurements.

ABSTRACT: Diverse tissues in vivo present varying degrees of confinement, constriction, and compression to migrating cells in both homeostasis and disease. The nucleus in particular is subjected to external forces by the physical environment during confined migration. While many systems have been developed to induce nuclear deformation and analyze resultant functional changes, much remains unclear about dynamic volume regulation in confinement due to limitations in time resolution and difficulty imaging in PDMS-based microfluidic chips. Standard volumetric measurement relies on confocal microscopy, which suffers from high phototoxicity, slow speed, limited throughput, and artifacts in fast-moving cells. To address this, we developed a form of double fluorescence exclusion microscopy, designed to fun

SUBMITTER: Li Y 

PROVIDER: S-EPMC11670245 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature