SPP1+ macrophages promote head and neck squamous cell carcinoma progression by secreting TNF-α and IL-1β.

ABSTRACT:

Background

Head and neck squamous cell carcinoma (HNSCC) is a very aggressive disease characterized by a heterogeneous tumor immune microenvironment (TIME). Tumor-associated macrophages (TAMs) constitute the major innate immune population in the TIME where they facilitate crucial regulatory processes that participate in malignant tumor progression. SPP1 + macrophages (SPP1 + Macs) are found in many cancers, but their effects on HNSCC remain unknown. This study aimed to identify and validate the role and function of SPP1 + Macs in the malignant progression of HNSCC.

Methods

In this study, we applied single-cell RNA sequencing (scRNA-seq) analyses of paired tumor and normal tissues from 5 HNSCC patients to identify tumor-specific SPP1 + Macs. RT-qPCR and multiplex immunohistoc

SUBMITTER: Liu C 

PROVIDER: S-EPMC11670405 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature