Ectopic expression of LONELY GUY7 in epidermis of internodal segments for de novo shoot regeneration without phytohormone treatment in ipecac.

ABSTRACT: In many plant species, the application of exogenous phytohormones is crucial for initiating de novo shoot regeneration. However, ipecac [Carapichea ipecacuanha (Brot) L. Andersson] has a unique ability to develop adventitious shoots on the epidermis of internodal segments without phytohormone treatment. This characteristic allows us to evaluate the effects of endogenous phytohormones in this species. Here, we showed that the presence of the pith, including vascular bundles in the internodal segment, is required to activate both endogenous cytokinin (CK) biosynthesis and adventitious shoot formation. Adventitious shoots were mainly formed in the apical region of internodal segments, where the CK biosynthesis genes ISOPENTENYL TRANSFERASE 3 (CiIPT3) and LONELY GUY 7 (CiLOG7) were spontaneous

SUBMITTER: Okazaki K 

PROVIDER: S-EPMC11670440 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature