ITGB3 is reduced in pregnancies with preeclampsia and its influence on biological behavior of trophoblast cells.

ABSTRACT:

Background

Preeclampsia (PE) is a serious pregnancy complication associated with impaired trophoblast function. Integrin β3 (ITGB3) is a cell adhesion molecule that plays a role in cell movement. The objective of this study was to identify the biological function and expression level of ITGB3 in PE.

Methods

Cell proliferation, migration, invasion, adhesion, and apoptosis were estimated by CCK8 assay, transwell, scratch assays, and flow cytometry, respectively. The expression levels of ITGB3 were determined by qRT-PCR, western blot, and immunohistochemistry (IHC). Co-immunoprecipitation and Alphafold-Multimer protein complex structure prediction software were employed to identify the molecules that interact with ITGB3.

Results

Cell functional experiments conducted on H

SUBMITTER: Li C 

PROVIDER: S-EPMC11670450 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature