Phenotypic Plasticity During Organofluorine Degradation Revealed by Adaptive Evolution.

ABSTRACT: A major factor limiting the biodegradation of organofluorine compounds has been highlighted as fluoride anion toxicity produced by defluorinating enzymes. Here, two highly active defluorinases with different activities were constitutively expressed in Pseudomonas putida ATCC 12633 to examine adaption to fluoride stress. Each strain was grown on α-fluorophenylacetic acid as the sole carbon source via defluorination to mandelic acid, and each showed immediate fluoride release and delayed growth. Adaptive evolution was performed for each recombinant strain by serial transfer. Both strains adapted to show a much shorter lag and a higher growth yield. The observed adaptation occurred rapidly and reproducibly, within 50 generations each time. After adaption, growth with 50-70 mM α-fluorophenylac

SUBMITTER: O'Connor MR 

PROVIDER: S-EPMC11670473 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature