Single-cell RNA sequencing reveals the critical role of alternative splicing in cattle testicular spermatagonia.

ABSTRACT: Spermatogonial stem cells (SSCs) form haploid gametes through the precisely regulated process of spermatogenesis. Within the testis, SSCs undergo self-renewal through mitosis, differentiation, and then enter meiosis to generate mature spermatids. This study utilized single-cell RNA sequencing on 26,888 testicular cells obtained from five Holstein bull testes, revealing the presence of five distinct germ cell types and eight somatic cell types in cattle testes. Gene expression profiling and enrichment analysis were utilized to uncover the varied functional roles of different cell types involved in cattle spermatogenesis. Additionally, unique gene markers specific to each testicular cell type were identified. Moreover, differentially expressed genes in spermatogonia exhibited notable enrichm

SUBMITTER: Wang X 

PROVIDER: S-EPMC11670508 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature