Activated DRP1 promotes mitochondrial fission and induces glycolysis in ATII cells under hyperoxia.

ABSTRACT:

Backgroud

Recent studies have reported mitochondrial damage and metabolic dysregulation in BPD, but the changes in mitochondrial dynamics and glucose metabolic reprogramming in ATII cells and their regulatory relationship have not been reported.

Methods

Neonatal rats in this study were divided into model (FIO2:85%) and control (FIO2: 21%) groups. Lung tissues were extracted at 3, 7, 10 and 14 postnatal days and then conducted HE staining for histopathological observation. We assessed the expression of mitochondria dynamic associated proteins and glycolysis associated enzymes in lung tissues, primary ATII cells and RLE-6TN cells. Double immunofluorescence staining was used to confirm the co-localization of DRP1 and ATII cells. Real-time analyses of ECAR and OCR were performed

SUBMITTER: Sun T 

PROVIDER: S-EPMC11670517 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature