Characterisation and hierarchy of the spermatogonial stem cell compartment in human spermatogenesis by spectral cytometry using a 16-colors panel.

ABSTRACT: About one in six couples experience fertility problems, and male infertility accounts for about half of these cases. Spermatogenesis originates from a small pool of spermatogonial stem cells (SSCs), which are of interest for the treatment of infertility but remain poorly characterised in humans. Using multiparametric spectral flow cytometric analysis with a 16-colours (16-C) panel of cell markers, we identify novel markers of SSCs and provide insights into unravelling and resolving the heterogeneity of the human spermatogonial cells. This 16-C panel of markers allowed the identification of a primitive SSCs state with the β2M^-CD51/61^-ITGA6⁺SSEA4⁺TSPAN33⁺THY1⁺CD9^medEPCAM^medCD155⁺CD148⁺

SUBMITTER: Lapoujade C 

PROVIDER: S-EPMC11671462 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature