Single-cell transcriptomes of dissecting the intra-tumoral heterogeneity of breast cancer microenvironment.

ABSTRACT:

Objective

To investigate the mechanism by which heterogeneity in breast cancer developed and acted in single-cell transcriptomes.

Methods

The composition of breast cancer based on the single-cell transcriptomes of 54,055 high-quality cells from clinical specimens of 4 malignant and 4 non-malignant patients were investigated.

Results

We identified six common expression programs and six subtype-specific expression programs form malignant epithelial cells. The expression program of malignant epithelial cells exhibited activated EMT (Epithelial Mesenchymal Transition) in TME, which might indicate EMT intervention have a general therapeutic effect on various subtypes. Gene set enrichment analysis (GSEA) based on the top 50 highly NMF (non-negative matrix factorization) sco

SUBMITTER: Chen P 

PROVIDER: S-EPMC11671554 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature