SnRNA-seq of long-preserved FFPE samples from colorectal liver metastasis lesions with diverse prognoses.

ABSTRACT: Differences in prognostic outcomes are prevalent in patients with colorectal cancer liver metastases. Comparative analysis of tissue samples, particularly applying single-cell transcriptome sequencing technology, can provide a deeper understanding of potential impacting factors. However, long-term monitoring for prognosis determination necessitates extended preservation of tissue samples using formalin-fixed and paraffin-embedded (FFPE) treatments, which can cause substantial RNA degradation, presenting challenges to single-cell or single-nucleus sequencing. In this study, employing snRandom-seq, a single-nucleus RNA sequencing (snRNA-seq) technology specifically for FFPE samples, we tested multiple lesion samples from 18 distinctive colorectal cancer liver metastasis cases with diverse pr

SUBMITTER: Chen H 

PROVIDER: S-EPMC11671580 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature