A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean.

ABSTRACT: Efficient genotype-independent transformation and genome editing are highly desirable for plant biotechnology research and product development efforts. We have developed a novel approach to enable fast, high-throughput, and genotype-flexible Agrobacterium-mediated transformation using the important crop soybean as a test system. This new method is called GiFT (genotype-independent fast transformation) and involves only a few simple steps. The method uses germinated seeds as explants, and DNA delivery is achieved through Agrobacterium infection of wounded explants as in conventional in vitro-based methods. Following infection, the wounded explants are incubated in liquid medium with a sublethal level of selection and then transplanted directly into soil. The transplanted seedlings are then

SUBMITTER: Zhong H 

PROVIDER: S-EPMC11671754 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature