Optimization of Cell Membrane Purification for the Preparation and Characterization of Cell Membrane Liposomes.

ABSTRACT: Cell membrane nanoparticles have attracted increasing interest in nanomedicine because they allow to exploit the complexity of cell membrane interactions for drug delivery. Several methods are used to obtain plasma membrane to generate cell membrane nanoparticles. Here, an optimized method combining nitrogen cavitation in isotonic buffer and sucrose gradient fractionation is presented. The method allows to obtain cell membrane fractions of high purity from both suspension and adherent cells. Comparison with other common methods for membrane extraction, where mechanical lysis using cell homogenizers is performed in isotonic or hypotonic buffers, shows that the optimized procedure yields high purity membrane in a robust and reproducible way. Procedures to mix the purified membrane with synth

SUBMITTER: de Weerd S 

PROVIDER: S-EPMC11671854 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature