Revealing the Mechanism Underlying 3D-AFM Imaging of Suspended Structures by Experiments and Simulations.

ABSTRACT: The invention of 3D atomic force microscopy (3D-AFM) has enabled visualizing subnanoscale 3D hydration structures. Meanwhile, its applications to imaging flexible molecular chains have started to be experimentally explored. However, the validity and principle of such imaging have yet to be clarified by comparing experiments and simulations or cross-observations with an alternative technique. Such studies are impeded by the lack of an appropriate model. Here, this difficulty is overcome by fabricating 3D carbon nanotube (CNT) structures flexible enough for 3D-AFM, large enough for scanning electron microscopy (SEM), and simple enough for simulations. SEM and 3D-AFM observations of the same model provide unambiguous evidence to support the possibility of imaging overlapped nanostructures, su

SUBMITTER: Alam MS 

PROVIDER: S-EPMC11672172 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature