Attenuation of neutrophil adhesion and formation of neutrophil extracellular traps by pooled human immune globulins.

ABSTRACT:

Introduction

This study investigated the efficacy of pooled human immune globulins (Flebogamma^® DIF) to combat the formation of neutrophil extracellular traps (NETs) and NETosis, along with neutrophil adhesion to corneal epithelial cells in response to dry eye disease relevant stimuli.

Methods

Human neutrophils were isolated by bead-based immunomagnetic depletion of non-target cells from human whole blood. NETosis was induced using phorbol 12-myristate 13-acetate (PMA) or anti-citrullinated histone 4 R3 antibody (H4R3 ACPA). Extracellular DNA was used as a surrogate biomarker of NETosis, and it was quantified using a 96-well, plate reader-based fluorescent assay and by confocal microscopy in 8-well chambers using the DNA dye, SYTOX^TM Green. Neutrophils

SUBMITTER: Rao VR 

PROVIDER: S-EPMC11672200 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature