Proliferative potential and angiogenic characteristics of blood outgrowth endothelial cells derived from middle-aged and older adults.

ABSTRACT:

Objectives

Autologous blood outgrowth endothelial cells (BOECs) have been proposed to induce therapeutic angiogenesis for treating cardiovascular diseases (CVDs). The aim of the present study was to investigate the proliferative potential and angiogenic characteristics of BOECs among middle-aged and older adults, the population particularly susceptible to CVDs.

Methods

BOECs were isolated from 48 peripheral blood samples of subjects aged 56 ± 4 years. The cells were then distinguished based on their proliferative abilities, and their phenotype, tube formation capacity, and migratory activity were compared using immunofluorescence staining, flow cytometry, tube formation assay, and wound healing assay, respectively. Correlations between demographic, clinical, and dietary para

SUBMITTER: Xia XJ 

PROVIDER: S-EPMC11672348 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature