An Improved <i>Theileria parva</i> Sporozoite Seroneutralization Assay for the Identification of East Coast Fever Immune Correlates.

ABSTRACT:

Background

Immune correlates of protection are ideal tools to predict treatment or vaccine efficacy. However, the accuracy of the immune correlate and the capability to robustly predict the outcome of a vaccine candidate are determined by the performance of the in vitro immunoassay used. Several Theileria parva sporozoite seroneutralization assays have previously been used to assess antibody functional activities; however, a common limitation has been the need for fresh material, target cells and sporozoites, and operator-to-operator bias. An improved assay represents a positive step toward overcoming challenges associated with variability and it might provide a more reliable means of establishing an immune correlate with protection after sub-unit vaccine administration.

SUBMITTER: Chege H 

PROVIDER: S-EPMC11672397 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature