Machine Learning-Assisted SERS Reveals the Biochemical Signature of Enhanced Protein Secretion from Surface-Modified Magnetic Nanoparticles.

ABSTRACT: This study introduces a novel investigation of the interaction between Komagataella phaffii cells and iron oxide-based magnetic nanoparticles (Fe₃O₄ MNPs) via protein secretion and machine learning (ML)-assisted surface-enhanced Raman scattering (SERS). For the first time, we produced Fe₃O₄, Fe₃O₄@PEG, Fe₃O₄@PEI_10kDa, and Fe₃O₄@PEI_25kDa MNPs by a one-pot coprecipitation reaction. The addition of polymers to the reaction conditions significantly affected the shape, surface charge, size, and size distribution of the MNPs. The surface modification of MNPs is effectively accomplished using polyethylenimine (PEI), and the ζ-potential values of the MNPs excee

SUBMITTER: Dagci I 

PROVIDER: S-EPMC11672479 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature