Examining the Relationship between Aptamer Complexity and Molecular Discrimination of a Low-Epitope Target.

ABSTRACT: Aptamers are oligonucleotide-based affinity reagents that are increasingly being used in various applications. Systematic evolution of ligands by exponential enrichment (SELEX) has been widely used to isolate aptamers for small-molecule targets, but it remains challenging to generate aptamers with high affinity and specificity for targets with few functional groups. To address this challenge, we have systematically evaluated strategies for optimizing the isolation of aptamers for (+)-methamphetamine, a target for which previously reported aptamers have weak or no binding affinity. We perform four trials of library-immobilized SELEX against (+)-methamphetamine and demonstrate that N30 libraries do not yield high-quality aptamers. However, by using a more complex N40 library design, stringen

SUBMITTER: Wang L 

PROVIDER: S-EPMC11672540 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature