Molecular Decoration and Unconventional Double Bond Migration in Irumamycin Biosynthesis.

ABSTRACT: Irumamycin (Iru) is a complex polyketide with pronounced antifungal activity produced by a type I polyketide (PKS) synthase. Iru features a unique hemiketal ring and an epoxide group, making its biosynthesis and the structural diversity of related compounds particularly intriguing. In this study, we performed a detailed analysis of the iru biosynthetic gene cluster (BGC) to uncover the mechanisms underlying Iru formation. We examined the iru PKS, including the domain architecture of individual modules and the overall spatial structure of the PKS, and uncovered discrepancies in substrate specificity and iterative chain elongation. Two potential pathways for the formation of the hemiketal ring, involving either an olefin shift or electrocyclization, were proposed and assessed u

SUBMITTER: Alferova VA 

PROVIDER: S-EPMC11672594 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature