CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.

ABSTRACT: The growing demand for sustainable platforms for biomolecule manufacturing has fuelled the development of plant-based production systems. Agroinfiltration, the current industry standard, offers several advantages but faces limitations for large-scale production due to high operational costs and batch-to-batch variability. Alternatively, here, we describe the CuBe system, a novel bean yellow dwarf virus (BeYDV)-derived conditional replicative expression platform stably transformed in Nicotiana benthamiana and activated by copper sulphate (CuSO₄), an inexpensive and widely used agricultural input. The CuBe system utilizes a synthetic circuit of four genetic modules integrated into the plant genome: (i) a replicative vector harbouring the gene of interest (GOI) flanked by cis-actin

SUBMITTER: Garcia-Perez E 

PROVIDER: S-EPMC11672746 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature