Microbiome and metabolome analysis in smoking and non-smoking pancreatic ductal adenocarcinoma patients.

ABSTRACT:

Background

Smoking is a significant risk factor for pancreatic ductal adenocarcinoma (PDAC). This study aimed to investigate the effects of smoking on the pancreatic microbiome and metabolome in resectable and unresectable male PDAC patients.

Methods

The pancreatic tissue samples were collected from resectable PDACs via surgery and unresectable PDACs via endoscopic ultrasound fine needle aspiration (EUS-FNA). Surgical samples obtained from 10 smoking and 6 non-smoking PDACs were measured by 16S ribosomal RNA (16S rRNA) gene sequencing and liquid chromatography-mass spectrometry (LC/MS). Fine needle aspiration (FNA) samples obtained from 20 smoking and 14 non-smoking PDACs were measured by 16S rRNA gene sequencing.

Results

From resectable to unresectable patients, the

SUBMITTER: Liang X 

PROVIDER: S-EPMC11673369 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature