Three-Color Protein Photolithography with Green, Red, and Far-Red Light.

ABSTRACT: Protein photolithography is an invaluable tool for generating protein microchips and regulating interactions between cells and materials. However, the absence of light-responsive molecules that allow for the copatterning of multiple functional proteins with biocompatible visible light poses a significant challenge. Here, a new approach for photopatterning three distinct proteins on a single surface by using green, red, and far-red light is reported. The cofactor of the green light-sensitive protein CarH is engineered such that it also becomes sensitive to red and far-red light. These new cofactors are shown to be compatible with two CarH-based optogenetic tools to regulate bacterial cell-cell adhesions and gene expression in mammalian cells with red and far-red light. Further, by incorpora

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC11673460 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature