Enhanced Biodiesel Production with Eversa Transform 2.0 Lipase on Magnetic Nanoparticles.

ABSTRACT: This research investigated the usefulness of magnetic iron oxide nanoparticles (Fe₃O₄) as a support to immobilize the lipase Eversa Transform 2.0 (ET 2.0) to obtain an active and stable biocatalyst, easily recoverable from the reaction medium for applications in the production of biodiesel. Biodiesel was an alternative fuel composed mainly of fatty acid esters with strong transesterification and esterification capabilities. The study focused on the esterification of oleic acid with ethanol to synthesize ethyl oleate. Magnetic nanoparticles were prepared by coprecipitation, then activated with glutaraldehyde and functionalized with γ-aminopropyltriethoxysilane (APTES). The optimal conditions for immobilizing ET 2.0 were pH 10, 25 mM sodium carbonate buffer, an enzymati

SUBMITTER: Dos Santos KM 

PROVIDER: S-EPMC11673575 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature