CPSF4-mediated regulation of alternative splicing of HMG20B facilitates the progression of triple-negative breast cancer.

ABSTRACT:

Background

Aberrant alternative splicing (AS) contributes to tumor progression. A crucial component of AS is cleavage and polyadenylation specificity factor 4 (CPSF4). It remains unclear whether CPSF4 plays a role in triple-negative breast cancer (TNBC) progression through AS regulation. In this study, our objective is to investigate the prognostic value of CPSF4 and pinpoint pivotal AS events governed by CPSF4 specifically in TNBC.

Methods

We examined the expression levels and prognostic implications of CPSF4 in patients diagnosed with TNBC through public databases. CPSF4-interacting transcripts, global transcriptome, and alternative splicing were captured through RNA immunoprecipitation sequencing (RIP-seq) and RNA sequencing (RNA-seq). The top 10 CPSF4-regulated alternati

SUBMITTER: Zhong G 

PROVIDER: S-EPMC11673668 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature