Ribosome engineering of Myxococcus xanthus for enhancing the heterologous production of epothilones.

ABSTRACT:

Background

Ribosome engineering is a semi-empirical technique used to select antibiotic-resistant mutants that exhibit altered secondary metabolism. This method has been demonstrated to effectively select mutants with enhanced synthesis of natural products in many bacterial species, including actinomycetes. Myxobacteria are recognized as fascinating producers of natural active products. However, it remains uncertain whether this technique is similarly effective in myxobacteria, especially for the heterologous production of epothilones in Myxococcus xanthus.

Results

Antibiotics that target the ribosome and RNA polymerase (RNAP) were evaluated for ribosome engineering of the epothilone-producing strain M. xanthus ZE9. The production of epothilone was dramatically altered in di

SUBMITTER: Kang X 

PROVIDER: S-EPMC11673899 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature