An Unlabeled Electrochemical Immunosensor Uses Poly(thionine) and Graphene Quantum Dot-Modified Activated Marigold Flower Carbon for Early Prostate Cancer Detection.

ABSTRACT: The activated carbon from marigold flowers (MG) was used to make an unlabeled electrochemical immunosensor to determine prostate cancer. MG was synthesized by hydrothermal carbonization and pyrolysis. MG had a large surface area, was highly conductive, and biocompatible. MG modified with graphene quantum dots produced excellent electron transfer for grafting poly(thionine) (PTH). The amine group of PTH bonded with anti-prostate-specific antigen (Anti-PSA) via glutaraldehyde, forming a layer that improved electron transfer. The binding affinity of the immunosensor, presented as the dissociation constant (Kd), was calculated using the Langmuir isotherm model. The results showed that a lower Kd value indicated greater antibody affinity. The immunosensor exhibited two different linear ranges u

SUBMITTER: Cotchim S 

PROVIDER: S-EPMC11674062 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature