BODIPY-Based Ratiometric Fluorescent Probe for Sensing Peroxynitrite in Inflammatory Cells and Tissues.

ABSTRACT: Peroxynitrite (ONOO^-) plays an important role in many physiological and pathological processes. Excessive ONOO^- in cells leads to oxidative stress and inflammation. However, precise monitoring of ONOO^- levels in specific organelles (e.g., mitochondria) is still lacking and urgently needed. Herein, we rationally designed a mitochondria-targeted ratiometric fluorescent probe, MOBDP-I, for imaging of ONOO^- in the mitochondria of inflammatory cells and model mice. This probe, MOBDP-I, was synthesized by conjugating a BODIPY fluorophore to a mitochondria-targeting moiety-indole-salt group by a carbon-carbon double bond (C=C). In the presence of ONOO^-, the C=C bond between the BODIPY backbone and the indole-salt group was oxidized and broken

SUBMITTER: Wu Q 

PROVIDER: S-EPMC11674116 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature