Development of a Novel Colorimetric pH Biosensor Based on A-Motif Structures for Rapid Food Freshness Monitoring and Spoilage Detection.

ABSTRACT: Accurate methods for assessing food freshness through colorimetric pH response play a critical role in determining food spoilage and ensuring food quality standards. This study introduces a novel unlabeled DNA sequence, poly-dA₂₀, designed to exploit the colorimetric properties of both the single strand and the fold-back A-motif structure in conjunction with gold nanoparticles (AuNPs) under varying pH conditions. When exposed to storage temperatures of 4 °C and 25 °C, the color variations in the AuNP solution, influenced by pH level changes in mutton and sea bass samples' different storage periods, are easily discernible to the naked eye within a minute. The ratio of UV absorption values at 527 nm and 700 nm (A₅₂₇/A₇₀₀) demonstrates a strong linear correlat

SUBMITTER: Wang J 

PROVIDER: S-EPMC11674138 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature