Antimicrobial activity and synergistic effect of phage-encoded antimicrobial peptides with colistin and outer membrane permeabilizing agents against Acinetobacter baumannii.

ABSTRACT:

Background

Acinetobacter baumannii poses a significant public health threat. Phage-encoded antimicrobial peptides (AMPs) have emerged as promising candidates in the battle against antibiotic-resistant A. baumannii.

Methods

Antimicrobial peptides from the endolysin of A. baumannii bacteriophage were designed from bacteriophage vB_AbaM_PhT2 and vB_AbaAut_ChT04. The peptides' minimum inhibitory concentration (MIC) and the synergistic effect of peptides with outer membrane-permeabilizing agents and colistin were determined. Cytotoxicity effects using HepG2 cell lines were evaluated for 24 h with various concentrations of peptides. Biofilm eradication assay was determined using the MIC concentration of each peptide. Galleria mellonella infection assay

SUBMITTER: Rothong P 

PROVIDER: S-EPMC11674141 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature