Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET Reversion.

ABSTRACT: Accurate and selective monitoring of thiamine levels in multivitamin supplements is essential for preventing deficiencies and ensuring product quality. To achieve this, a Förster resonance energy transfer (FRET) system using carbon dots (CDs) as energy donors and citrate-stabilized silver nanoparticles (AgNPs) as energy acceptors was developed. The aqueous synthesis of AgNPs using microwave irradiation was optimized to obtain efficient plasmonic nanoparticles for FRET applications, targeting maximal absorbance intensity, stability, and wavelength alignment. Using a central composite orthogonal design (CCOD), the optimal conditions were identified as a 12.5 min microwave reaction time, a Ag molar ratio of 0.72, and a pH of 8.28. The FRET sensing scheme was applied for thiamine determination

SUBMITTER: Castro RC 

PROVIDER: S-EPMC11674425 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature