SARS-CoV-2 membrane protein induces neurodegeneration via affecting Golgi-mitochondria interaction.

ABSTRACT:

Background

Neurological complications are a significant concern of Coronavirus Disease 2019 (COVID-19). However, the pathogenic mechanism of neurological symptoms associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is poorly understood.

Methods

We used Drosophila as a model to systematically analyze SARS-CoV-2 genes encoding structural and accessory proteins and identified the membrane protein (M) that disrupted mitochondrial functions in vivo. The M protein was stereotaxically injected to further assess its effects in the brains of wild-type (WT) and 5 × FAD mice. Omics technologies, including RNA sequencing and interactome analysis, were performed to explore the mechanisms of the effects of M protein both in vitro and in vivo.

Results<

SUBMITTER: Wang F 

PROVIDER: S-EPMC11674522 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature