Development of a bacterial gene transcription activating strategy based on transcriptional activator positive feedback.

ABSTRACT:

Introduction

Transcription of biological nitrogen fixation (nif) genes is activated by the NifA protein which recognizes specific activating sequences upstream of σ⁵⁴-dependent nif promoters. The large quantities of nitrogenase which can make up 20% of the total proteins in the cell indicates high transcription activating efficiency of NifA and high transcription level of nifHDK nitrogenase genes.

Objectives

Development of an efficient gene transcription activating strategy in bacteria based on positive transcription regulatory proteins and their regulating DNA sequences.

Methods

We designed a highly efficient gene transcription activating strategy in which the nifA gene was placed directly downstream of its regulating sequences. The NifA protein binds its

SUBMITTER: Yu G 

PROVIDER: S-EPMC11674765 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature