Unraveling the Mechanism of Impaired Osteogenic Differentiation in Osteoporosis: Insights from <i>ADRB2</i> Gene Polymorphism.

ABSTRACT: Osteoporosis is characterized by increased resorption and decreased bone formation; it is predominantly influenced by genetic factors. G-protein coupled receptors (GPCRs) play a vital role in bone homeostasis, and mutations in these genes are associated with osteoporosis. This study aimed to investigate the impact of single nucleotide polymorphism (SNP) rs1042713 in the ADRB2 gene, encoding the beta-2-adrenergic receptor, on osteoblastogenesis. Herein, using quantitative polymerase chain reaction, western immunoblotting, immunofluorescence assays, and flow cytometry, we examined the expression of ADRB2 and markers of bone matrix synthesis in mesenchymal stem cells (MSCs) derived from osteoporosis patient (OP-MSCs) carrying ADRB2 SNP in comparison with MSCs from healthy donor

SUBMITTER: Krasnova O 

PROVIDER: S-EPMC11674950 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature