Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock.

ABSTRACT:

Background/objectives

Actin plays a crucial role not only in the cytoplasm, but also in the nucleus, influencing various cellular behaviors, including cell migration and gene expression. Recent studies reveal that nuclear actin dynamics is altered by cellular stresses, such as DNA damage; however, the effect of heat shock on nuclear actin dynamics, particularly in the nucleolus, remains unclear. This study aims to elucidate the contribution of nucleolar actin to cellular responses under heat shock conditions.

Methods

Nuclear actin dynamics in response to heat shock were investigated using nAC-GFP, a GFP-tagged actin chromobody, to visualize nuclear actin in HeLa cells. Bioinformatic analyses were also performed.

Results

Heat shock induced the reversible assembly of nA

SUBMITTER: Taniguchi S 

PROVIDER: S-EPMC11675300 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature