Identification and Expression Analysis of Lipoxygenase Gene in Bitter Gourd (Momordica charantia).

ABSTRACT:

Background

Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role in the synthesis of fruit aroma volatiles.

Methods

LOX gene family members were identified in the whole genome database of bitter gourd and analyzed bioinformatically. An RT-qPCR was used to analyze the expression differences in different tissues. Monoterpenes were determined by gas chromatography-mass spectrometry (GC-MS) technique.

Results

A total of 12 LOX gene family members were identified in the genome. The expression of LOX genes varied significantly among the tissues of roots, stems, leaves, flowers, fruits, seeds and tendrils. A total of 29 monoterpenes were detected in the fruits of five different

SUBMITTER: Ge H 

PROVIDER: S-EPMC11675646 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature