Cutaneous burn diminishes beneficial effect of intravenously administered mesenchymal stem cells on acute lung injury induced by smoke inhalation in sheep.

ABSTRACT:

Objective

To investigate effects of intravenously administered allogeneic mesenchymal stem cells (MSCs) on burn/smoke-induced lung injury.

Methods

Sheep were subjected to 40%, third-degree flame skin burn and smoke inhalation under deep anesthesia and analgesia. One-hour after injury, PlasmaLite A (control) or 200 million MSCs (treatment) were intravenously administered. Pulmonary oxygenation index, PaO₂/FiO₂ ratio, lung-lymph flow, and bloodless lung wet-to-dry weight ratio were measured. Distribution of MSCs and stromal cell-derived factor-1 (Sdf-1) protein level were determined in lung and skin tissues. Effects of burn exudate on MSCs migration were characterized.

Results

MSCs did not attenuate pulmonary dysfunction. The number of MSCs was sig

SUBMITTER: Baljinnyam T 

PROVIDER: S-EPMC11676003 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature