Effect of a Mating Type Gene Editing in <i>Lentinula edodes</i> Using RNP/Nanoparticle Complex.

ABSTRACT: Gene editing using CRISPR/Cas9 is an innovative tool for developing new mushroom strains, offering a promising alternative to traditional breeding methods that are time-consuming and labor-intensive. However, plasmid-based gene editing presents several challenges, including the need for selecting appropriate promoters for Cas9 expression, optimizing codons for the Cas9 gene, the unintended insertion of fragmented plasmid DNA into genomic DNA (gDNA), and regulatory concerns related to genetically modified organisms (GMOs). To address these issues, we utilized a Ribonucleoprotein (RNP) complex consisting of Cas9 and gRNA for gene editing to modify the A mating-type gene of Lentinula edodes. To overcome the challenges posed by the large size of the Cas9 protein, which limits its penetr

SUBMITTER: Kim M 

PROVIDER: S-EPMC11677008 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature